13C代谢流和定量代谢物组学研究团队
利用本实验室先进的微型反应器、快速取样装置和高效液相/气相质谱仪可以同时实现胞内代谢物浓度的测定和13C代谢流的计算，从而为以13C代谢流为核心的纵向组学整合技术打下坚实基础。并与宏观过程生物生理代谢参数OUR、CER、RQ等相结合分析，实验发酵过程的全局优化。
快速取样、淬灭和提取装置示意图
13C代谢流和定量代谢物组测定流程图
13C代谢流和定量代谢物组学在黑曲霉生产糖化酶过程中的应用
研究成果
1.Impacts of high β-galactosidase expression on central metabolism of recombinant Pichia pastoris GS115 using glucose as sole carbon source via13C metabolic flux analysis. Journal of Biotechnology, 2014, 187: 124-134.
2.Integrated isotope-assisted metabolomics and 13C metabolic flux analysis reveals metabolic flux redistribution for high glucoamylase production by Aspergillus niger. Microbial Cell Factories, 2015, 14: 147-160.
3.Enhanced L-lactic acid production in Lactobacillus paracasei by exogenous proline addition based on comparative metabolite profiling analysis. Applied Microbiology and Biotechnology, 2016, 100: 2301-2310.